ABSTRACT
Objective:To investigate the role and regulatory mechanism of miR-125b-1-3p in rotavirus replication.Methods:MA104 cells were infected with rotavirus after upregulation and down-regulation of miR-125b-1-3p, respectively. The expression of miR-125b-1-3p and the copy number of rotavirus were analyzed by RT-PCR. The effect of miR-125b-1-3p on the protein expression of rotavirus was analyzed by immunofluorescence. The expression of related proteins involved in the regulation of miR-125b-1-3p was analyzed by Western blot analysis.Results:After rotavirus infection, the expression level of miR-125b-1-3p was significantly up-regulated, the copy number of VP7 and NSP3 gene of rotavirus decreased after up-regulation of miR-125b-1-3p, and the copy number of VP7 and NSP3 gene of rotavirus was significantly increased after down-regulation of miR-125b-1-3p.The fluorescence number of rotavirus protein decreased after upregulation of miR-125b-1-3p expression level, and increased after down-regulation of miR-125b-1-3p expression level. The activity of PI3K/Akt pathway was inhibited 16 h after rotavirus infection, and the up-regulation of miR-125b-1-3p could inhibit the activation of PI3K/Akt pathway.Conclusions:MiR-125b-1-3p inhibits rotavirus replication by regulating the PI3K/Akt pathway. These results provide an experimental basis for exploring the specific regulatory mechanism between miR-125b-1-3p and PI3K/Akt pathway, and provide a target for anti-infection therapy of rotavirus.
ABSTRACT
Objective@#To observe the clinical effect of Xiayuxue decoction in the treatment of endometriosis.@*Methods@#88 patients with endometriosis were selected and randomly divided into two groups by drawing lots, with 44 cases in each group.The control group received danazol treatment, the observation group was given TCM Xiayuxue decoction.Before and after treatment, the serum endocrine hormones[serum estradiol (E2), testosterone(T), progesterone(P), luteinizing hormone(LH), follicle stimulating hormone(FSH)]contents and humoral immunity(C3, C4) levels, and the clinical effect were compared between the two groups.@*Results@#After treatment, the E2, T, P, LH, FSH levels of observation group were (109.47±20.67)ng/L, (441.23±120.14)ng/L, (15.11±4.13)g/L, (9.85±2.34)IU/L, (3.05±0.90)IU/, respectively, which of the control group were (100.22±17.45)ng/L, (500.47±110.24)ng/L, (15.34±4.20)g/L, (9.01±1.04)IU/L, (3.08±0.91)IU/, respectively, there were statistically significant differences between the two groups(t=2.27, 2.41, 0.26, 2.18, 0.16, all P<0.05). The C3, C4 levels of the observation group were (1.09±0.25)g/L, (0.52±0.10)g/L, respectively, which of the control group were (1.00±0.10)g/L, (0.77±0.46)g/L, respectively, there were statistically significant differences between the two groups(t=2.23, 3.52, all P<0.05). The total effective rate of the observation group was 95.45%, which was significantly higher than 79.55% of the control group (χ2=5.09, P<0.05).@*Conclusion@#Xiayuxue decoction in the treatment of endometriosis can improve the endocrine hormone and humoral immunity of patients, and the effect is excellent.
ABSTRACT
Objective To observe the clinical effect of Zhengan Xifeng decoction in the treatment of Parkinson's disease.Methods From January 2013 to January 2017,100 patients with Parkinson's disease in the People's Hospital of Dongping County were included in the study and they were randomly divided into two groups according to the digital table,with 50 cases in each group.The control group was treated with Madopar,the observation group was treated with Madopar combined with Zhengan Xifeng decoction.Mter 12 weeks of treatment,the clinical efficacy,incidence of adverse reactions,unified Parkinson's disease score,ADL score,cognitive function index of the two groups were compared.Results The total effective rate of the observation group was 92％ (46/50),which was significantly higher than 74％ (37/50) of the control group (x2 =5.741,P ＜ 0.05).The incidence rate of adverse reactions of the observation group was 6％ (3/50),which of the control group was 4％ (2/50),the difference was not statistically significant(x2 =0.211,P ＞ 0.05).After treatment,the unified Parkinson's disease score,ADL score,Montreal cognitive function score,mini mental state score in the observation group were (49.93 ± I0.86) points,(85.71 ± 14.53) points,(25.93 ± 2.76) points,(23.81 ± 4.12) points,respectively,which in the control group were (60.56 ± 11.49) points,(71.46 ± 14.02) points,(22.56 ± 2.65) points,(19.42 ±.4.05) points,respectively,there were statistically significant differences between the two groups (t =4.754,4.990,6.228,5.373.all P ＜ 0.05).Conclusion Zhengan Xifeng decoction in the treatment of Parkinson's disease can effectively improve the patients' cognitive function,daily life ability,alleviate the symptoms of Parkinson's disease.
ABSTRACT
Acute pancreatitis( AP)is a common gastrointestinal emergency,which comprises mild acute pancreatitis ( MAP),moderately severe acute pancreatitis( MSAP)and severe acute pancreatitis( SAP). Most patients with MAP and MSAP present as a mild,self-limiting disease with low morbidity and mortality,however,patients who suffer from SAP will experience serious local and/or systemic complications associated with high mortality. Therefore,early assessment of disease severity is important for clinical management. Procalcitonin( PCT),a pro-peptide of calcitonin,is an inflammatory mediator that can be used to differentiate bacterial and non-bacterial infection. Recently,the effect of PCT on AP has attracted more and more attentions. This article reviewed the advances in studies on PCT and AP.
ABSTRACT
Objective To establish and optimize AFLP reaction system used in providing necessary technique basis for genetic diversity analysis in Asarum sieboldii.Methods Leaves of A.sieboldii were used as experimental materials to analyze various essential elements of the whole process,such as quality of extracted DNA,time of restriction digest and ligation,concentration of Mg2+,primers and Taq DNA polymerase during PCR amplification etc.,so that the most optimal AFLP reaction system could be built up. Results The optimal AFLP reaction system of A.sieboldii has been constructed: in the genomic DNA extraction,mercaptoethanol being utilized and samples being incubated about 30 min at 65 ℃;the genomic DNA being digested by Trul Ⅰ and Pst Ⅰ for 3 h respectively;in PCR amplification,the final concentration of Mg2+ being 1.5 mmol/L and the volume of Taq DNA polymerase being 0.2 ?L.Conclusion The present reaction system for A.sieboldii is able to gain the favorable results of AFLP analysis and can be used in the genetic diversity research of the species.